The thermal stability of proteins can be used to assess ligand binding in living cells. We have generalized this concept by determining the ...
Tracking drugs in living cells. Drugs alter the thermal stability of proteins directly through compound binding or indirectly through changes in ...
Thermal shifts were also observed for downstream effectors of drug treatment. In live cells, dasatinib induced shifts in BCR-ABL pathway ...
In an alternative method, the cells were extracted first, and the extracts were treated with drug (“1”). For each condition, the cell or cell-extract ...
Request PDF | Proteomics. Tracking cancer drugs in living cells by thermal profiling of the proteome. | The thermal stability of proteins can be ...
Using thermal proteome profiling to track target and non-target thermal
Tracking cancer drugs in living cells by thermal profiling of the proteome. Science , 346(6205):1255784. [Link]. Berndt, J. D., & Wong, W. (2015). 2014: Signaling ...
The thermal stability of proteins can be used to assess ligand binding in living cells. We have generalized this concept by determining the thermal profiles of ...
Cellular Target Engagement in Drug Discovery
Thermal shifts were also observed for downstream effectors of drug treatment. In live cells, dasatinib induced shifts in BCR-ABL pathway proteins, including CRK/ ...